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Primary hippocampal neuron cultures are used to study Dihexa's proposed effects on dendritic spine density and synaptic protein expression, typically measuring morphological changes via fluorescence microscopy and quantifying synaptic marker expression (such as PSD-95 or synaptophysin) relative to untreated controls

Wu AL, Moye-Rowley WS (1994) GSH1 , which encodes -glutamylcysteine synthetase, is a target gene for yAP-1 transcriptional regulation