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At OD 600 of 0.5, cultures were infected with M13K07 helper phage by adding 5 L of 2 10 10 PFU/L stock to 25 mL cultures

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But B12 seems to be lost in modern, high-speed farming methods, so there appears to be a lot less B12 in meat and dairy than there used to be, resulting in a lot more people with B12 deficiency

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