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Semi-quantitative analysis of specific extracellular H + level (seH + ) was performed using the ImageJ software by measuring the area of the yellow region (adjust color threshold, make binary, and remove outliers) and the intensity of the yellow color (live histogram) and the root area and by applying the following formula: where A y is the area of the yellow region, I is the color intensity of the yellow region, and A r is the root area

Cells were cultured in high-glucose DMEM supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin at 37C in a humidified atmosphere containing 5% CO 2

10.1007/pl00008553 Parasitol