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If 1,000 mcg of peptide is dissolved in 2 mL of bacteriostatic water, the concentration is calculated like this: Concentration = Total peptide amount Total solution volume 1,000 mcg 2 mL = 500 mcg/mL This means each milliliter of solution contains 500 mcg of peptide

Briefly, the process proceeds with the mRNA synthesis of seleno-GPxs by transcription factors followed by in situ synthesis of Sec on a specific set of tRNA designated as tRNA [ser]Sec and its incorporation into the peptide sequence against the termination codon UGA within ribosomal machinery (Fig

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The resulting fractions were then re-suspended in buffer A (16 L) and analyzed on an Orbitrap Fusion mass spectrometer