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Briefly, cohorts of 3050 adult flies (approximately 50 mg of fresh tissue) were collected in Eppendorf tubes containing and transferred to 500 ul ice-cold 50 g/l perchloric acid solution containing 0.2 M boric acid and 10 uM gamma-Glu-Glu on ice

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4.3 Effects of melatonin on early embryonic development and oocyte quality 4.3.1 Effects on early embryonic development Melatonin affects both gamete formation and early embryo development