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3D cell culture models Most in vitro studies of ECD rely on 2D cell models where a monolayer of ECs is grown on tissue culture polystyrene (TCPs) plates [237]

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Early studies discovered a reduced incretin effect in individuals with type 2 diabetes following oral glucose intake 16,17,18

3.2 Bifidobacterium species: therapeutic and functional profiles The genus Bifidobacterium comprises a diverse group of probiotic bacteria critical for maintaining GI homeostasis and developing functional therapeutic applications ( Table 2 )