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however, it can lead to the worsening of neurological conditions in about one-third of Wilsons Disease patients who present with neurological symptoms, especially if they have concomitant chronic liver disease, leukopenia or thrombo- cytopenia

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Proteins were then precipitated by addition of 500 l methanol (pre-cooled at 80 C), 400 l LCMS-grade water, and 100 l chloroform, and the mixture was vortexed and centrifuged (10,000 g, 10 min, 4C) to afford a protein disc at the interface of CHCl3 and aqueous layers